Development of A Dual Real-time PCR for the Rapid Detection of Listeria monocytogenes

To establish a rapid assay for Listeria monocytogenes(LM) detection,a Real-time PCR method was developed targeting iap gene of LM.The results showed that the test for 15 bacteria strains,only LM was positive,indicated that the method had high specificity.In addition,the sensitivity of Real-time PCR was 6.5 CFU/mL.Stability and reproducibility of the test showed that the coefficient of variation for the same sample repeat the Ct values were less than 2%.Furthermore,a total of 3 positive samples for LM were detected from 139 clinical samples by the method,which was in accordance with the testing result by GB 478930-2010 standard detection protocol.Therefore,the Real-time PCR method provides a novel rapid,sensitive and good repeatability detection method for LM infection.     

Study on Fans Cooling of Dairy Cowshed with Free Stall in Northern Mountainous Region of Hebei in Summer

The present objective of the study was to investigate the effect of fans cooling on dairy cow in northern mountainous region of Hebei in summer.Two cowsheds with the same structure and feeding technics were used to investigate the effect of fans on indoor temperature and humidity, physiological parameters and milking performance of dairy cow.The results showed that the average ambient temperature in cowshed with fans was 1.5℃ lower than that of the control cowshed, and the average wind velocity was 0.49 m/s higher than that of the control cowshed, reaching to 0.66 m/s.Temperature-humidity index (THI) of cowshed with fans was significantly lower than that of the control shed during the period of fans running (P<0.05).Besides, the rectal temperature and respiration rate of dairy cows in cowshed with fans were significantly lower than of the control cowshed (P<0.05), and the milk yield per cow per day was 17.0% higher than that of the control cowshed (P<0.05), however, the milk fat, milk protein and milk sugar were no significant difference from the control (P>0.05).Overall, fans could decrease indoor environment, moderate heat stress and increase performance and health of dairy cows in northern mountainous region of Hebei.     

园林绿地刺吸害虫及其天敌时序动态及发生规律的研究

为有效保护园林绿地中的自然天敌,以园林植物刺吸害虫及其天敌为调查对象,采用黄板诱集法,研究了园林绿地刺吸害虫及其天敌时序动态及发生规律。结果表明:绿地中京枫多态毛蚜(Periphyllus diacerivorus)、栾多态毛蚜(P.koelreuteria)、居松长足大蚜(Cinara pinihabitans)和白皮松长足大蚜(C.bungeanae)的发生期早于桃粉大尾蚜(Hyalopterus arundimis),且5种蚜虫种群均在夏季高温和天敌共同作用下崩溃;瓢虫和寄生蜂类天敌对碧桃(Pruns perscial f.duplex)上桃粉大尾蚜及捕食螨对碧桃上山楂叶螨(Tetranychus viennensis)的跟随效应明显,而天敌对其它4种植物上蚜虫无明显跟随效应;施药虽可暂时降低阔叶植物上3种蚜虫的种群数量,却大量杀伤了害虫的天敌,停药后3种蚜虫仍爆发成灾,同时使6月后碧桃上山楂叶螨种群爆发和5月中旬后油松(Pinus tabuliformis)上针叶小爪螨(Oligonychus ununguis)种群数量较多。绿地中瓢虫、草蛉、食蚜蝇和寄生蜂类天敌的发生最高峰均位于5月下旬至6月下旬,除草蛉外,绿地中的瓢虫、食蚜蝇和寄生蜂类天敌在7—8月种群数量均极低。草蛉喜欢在五角枫(Acer mono)上栖息或搜寻寄主。     

Sintering of Magnetite Concentrates Without Carbon

With the goal of the sintering of magnetite concentrates without carbon,the effect of quality,inert atmosphere and Fe_3O_4 addition on microwave sintering of magnetite concentrates is studied.It is shown that: With the same basicity(CaO/SiO_2=2.5) and microwave heating condition(1000W,2.45GHz,20min),the centre temperature decreaseds with a increasing sample mass;There is no great difference between with and without inert atmosphere in microwave sintering of magnetite in a same microwave heating condition.When 100g sample(CaO/SiO_2=2.5) is heated by microwave irradiation(1000W,2.45GHz,20min),the more Fe_3O_4 is added,the less liquid phase is produced,which is bad for microwave sintering of magnetite concentrate.     

Phase Transformation Diffusion Bonding of Titanium Alloy to Stainless Steel

Jointing of titanium alloy and stainless steel is very often in the fields like aviation, chemical plants etc. The combination of materials has the advantages of both titanium alloy and stainless steel and will reduce the consumption of titanium resource. The phase transformation diffusion bonding of TA17 titanium alloy to 0CriSNigTi stainless steel are carried out on Gleeble - 1500D. The effcet of maximum thermal cycle temperature, the tensile strength, the fracture surface and microstruture of the joints are investigated. The results show that TiFe and TiFe2 intermetallics and some solid solutions are formed along the interface, when maximum thermal cycle temperature increase, the area proportion of intermetallics in the fracture surface increase, which are detrimental to the mechanical properties of the transition joints.     

Design of Data Acquisition System for Vehicle Based on CAN Bus

To the question that a meter is correspond with a set of lead and a sensor,this paper presents a design of data acquisition system for vehicle based on CAN bus.In this way,the combinatorial instrument is only connected by a bus unit.Author introduces the data acquisition structure chart and the CAN interface,analyzes the software by program flow chart,and shows it's application for vehicle virtual instrument.A method of calculating vehicle speed,engine rotate speed and the course of development mileage are also given.     

喷雾机器人远程控制系统的设计与试验研究

结合喷雾机器人作业环境的要求,开发出基于客户端/服务器模式的远程控制平台.该平台实现了通过无线网络实时传输各类数据和控制命令,客户端再现了机器人的工作环境和状态.在此基础上进行了系统性能测试和轨迹测试.试验结果表明:为了保证良好的操控性,机器人所处区域信号强度必须大于30%,操作者根据反馈信息判断机器人是否偏离预定路径,并在偏离时给予有效的纠正,为解决喷雾机器人重喷、漏喷的问题提供了一种方法.     

离心泵节能技术工作的改进措施

针对离心泵节能问题,从设计、制造、配套等技术角度进行了阐述分析,提出可通过CAD/CFD技术的应用、传统设计方法的提高和改进、CAM技术在泵制造过程中的应用、提高铸造技术水平、调节转速以及提高离心泵选型的设计水平等措施来实现高效节能的目标。并通过分析目前存在的制约节能工作开展的问题,提出改进离心泵节能工作的实施办法。     

白口铸铁机械性能研究及在农机制造中的应用(I)

为了提高白口铸铁的冲击韧性等机械性能,扩大其在农机具上的应用范围,采用BZ变质剂对白口铸铁进行变质浇铸,并研究了变质剂对白口铸铁中碳化物的断网效果。试验结果表明,变质剂对于含碳量在3.2%以下、含铬量在12%以下的白口铸铁具有较好的断网效果。